Promoter Cloning of Human SETDB1 Gene Utilizing Bioinformatic Programs
نویسندگان
چکیده
منابع مشابه
Molecular cloning and characterization of human homeobox gene Nkx3.1 promoter.
Nkx3.1 is a prostate-specific homeobox gene related strongly to prostate development and prostate cancer. To study its regulation of transcription, 1.06 kb 5' flanking region of Nkx3.1 gene and its 5' deletion mutants (861, 617, 417 and 238 bp) were obtained by PCR and cloned into pGL(3)-basic, a promoter-less luciferase reporter vector, to examine their promoter activities driving the reporter...
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In mammals, RNA interference is primarily a post-transcriptional mechanism. Evidence has accumulated for additional role in transcriptional gene silencing (TGS) but the question for a good paradigm for small interfering antigene RNA (agRNA)-induced chromatin modification remains unanswered. Here, we show that SETDB1, a histone H3-lysine 9 (H3K9)-specific methyltransferase, cooperates with Argon...
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ژورنال
عنوان ژورنال: Journal of Life Science
سال: 2014
ISSN: 1225-9918
DOI: 10.5352/jls.2014.24.1.1